Application of pH and pO2 Probes During Bacillus caldolyticus Fermentation: An Additional Approach for Improving a Feeding Strategy
نویسندگان
چکیده
My colleague Wolf-Dieter Deckwer and I spent very productive time as PhD students in the Institut für Technische Chemie, Technische Universität, Berlin, Germany between 1971 and 1976 working together on modelling of hydrodynamics, mass and heat transfer in bubble columns. During this time we published some very good papers especially in the area of absorption and reaction of isobutene in sulphuric acid. Later, we both moved into Biotechnology and met frequently at diverse meetings and conferences. Wolf-Dieter’s analysis and discussions during these politically turbulent times in West-Berlin were distinctive for me and for the whole generation of PhD students in our institute at this time. Friendly contacts with him and his family lasted over 30 years. I am glad and proud to belong to this group of (formerly) young scientists. M. K. Popovic
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-Amylase Production in Fed Batch Cultivation of Bacillus caldolyticus: An Interpretation of Fermentation Course Using 2-D Gel Electrophoresis
The conditions for increased production of thermostable -amylase from Bacillus caldolyticus DSM 405 were investigated. Preliminary experiments in batch shake flasks led to an optimized initial cultivation medium. Shake flask experiments in extended-batch and in fed-batch mode of operation indicated that the -amylase production was enhanced by continuous feeding of starch. The activity of the -a...
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